ASSIGNMENT 2

 due date:  2^nd Oct 2010

1)      What is the difference between fluorescence and phosphorescence?  Are all molecules fluorescent in nature?

2)      In the context of fluorescence spectroscopy, what is internal conversion and intersystem crossing?

3)      In FRET, if the distance between the donor and acceptor is increased by 2, by what percentage does the energy transfer efficiency change?

4)      In Fluorescence lifetime spectroscopy, intensity is plotted against time. Draw a schematic lifetime plot for (a) unquenched (b) quenched molecule.

5)      In FCS, what is auto correlation function and how can it be utilized to study dynamics of a fluorescent molecule?

6)      Among fluorescence and Raman spectroscopy, which method is dependent on incident wavelength?

7)      Raman spectroscopy and infra-red spectroscopy can be used to probe vibrational energy states of molecules. What is the difference between these two techniques?

8)      What do you mean by non-linear optical spectroscopy?

BIO322 lecture slides

Link to lecture slides

OPEN SOURCE ASSIGNMENT - BIO322

LINK to the short essays written by BIO322 students

Ion channels, ion pumps, neuron etc.

This below youtube video depicts the selectivity filtering of ions through channels




The below video shows the functioning of Sodium - Potassium pump (movement against concentration gradient !)



The concept of active and passive transport is clearly explained in the following video



Anatomy of a neuron is explained in the below video



The concept of action potential is explained below



Now comes the nerve impulse....






Neural Synapse

Flagella, Cilia and more...

The following videos (from YOUTUBE) show Brownian motion, and dynamics of Flagella and Cilia

Molecular motors

Molecular motors are fascinating. They move inside and outside cells, and their movement can be experimentally tracked. Understanding the motion of molecular motors is important to understand many aspects of functioning of cells, such as traffic, energy budget etc. Below YOUTUBE links explain the functioning of KINESIN, a molecular motor.