due date: 2nd Oct 2010
1) What is the difference between fluorescence and phosphorescence? Are all molecules fluorescent in nature?
2) In the context of fluorescence spectroscopy, what is internal conversion and intersystem crossing?
3) In FRET, if the distance between the donor and acceptor is increased by 2, by what percentage does the energy transfer efficiency change?
4) In Fluorescence lifetime spectroscopy, intensity is plotted against time. Draw a schematic lifetime plot for (a) unquenched (b) quenched molecule.
5) In FCS, what is auto correlation function and how can it be utilized to study dynamics of a fluorescent molecule?
6) Among fluorescence and Raman spectroscopy, which method is dependent on incident wavelength?
7) Raman spectroscopy and infra-red spectroscopy can be used to probe vibrational energy states of molecules. What is the difference between these two techniques?
8) What do you mean by non-linear optical spectroscopy?
No comments:
Post a Comment